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Candidate Genes of molecular regulation of Vascular Cambium as possible targets using modified CRIPSR/Cas9 system using flowering stimulation and precision lightingCRISPR/Cas9 has several major advantages over previous transgenic-based approaches and can work alongside conventional breeding programs by directly improving known yield-related loci or genes. We have a modified CRISPR/Cas9 system that promotes early flowering in plants. We are now testing this technology in Arabidopsis thaliana with the aim to accelerate breeding in arboreus species
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Acceleration of CRISPR/Cas9 breeding using flowering stimulation and precision lightingAs a renewable resource, the demand for wood products is expected to continue to increase into the future. Compared to annual crop plants, direct genetic modification of trees species has gained little attention. The newly developed gene editing technologies, such CRISPR/Cas9, increase the potential for the modification of species. Together with precision lighting, we have managed to regulate the acceleration of flowering time to get viable flowers
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C2Pe: a new qPCR algorithmWe propose a novel algorithm that overcomes shortcomings in previously published methods. C2Pe considers 2 phases of a reaction, and the PCR efficiency to determine the Ct value. We have also incorporated revised statistics to improve the robustness of the analysis and constructed a web-based interface to give a straightforward, streamlined qPCR experience.
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