Candidate Genes of molecular regulation of Vascular Cambium as possible targets using modified CRIPSR/Cas9 system using flowering stimulation and precision lighting
CRISPR/Cas9 has several major advantages over previous transgenic-based approaches and can work alongside conventional breeding programs by directly improving known yield-related loci or genes. We have a modified CRISPR/Cas9 system that promotes early flowering in plants. We are now testing this technology in Arabidopsis thaliana with the aim to accelerate breeding in arboreus species
Acceleration of CRISPR/Cas9 breeding using flowering stimulation and precision lighting
As a renewable resource, the demand for wood products is expected to continue to increase into the future. Compared to annual crop plants, direct genetic modification of trees species has gained little attention. The newly developed gene editing technologies, such CRISPR/Cas9, increase the potential for the modification of species. Together with precision lighting, we have managed to regulate the acceleration of flowering time to get viable flowers
Is the optimal light really an optimal light for plants?
In growth rooms, when artificial light is required, normally fluorescence tubes and, recently, a new type of white LEDs are chosen. But is that the right choice?
CRISPR/Cas9 and precision light
The overarching goal of this research is to develop methods to accelerate the production of homozygous, transgene-free, plants with mutations in genes of interest.
C2Pe: a new qPCR algorithm
We propose a novel algorithm that overcomes shortcomings in previously published methods. C2Pe considers 2 phases of a reaction, and the PCR efficiency to determine the Ct value. We have also incorporated revised statistics to improve the robustness of the analysis and constructed a web-based interface to give a straightforward, streamlined qPCR experience.